MIA PaCa-2 人胰腺癌細胞 (STR)

簡要描述：CRL-1420 MIA PaCa-2 人胰腺癌細胞
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詳細介紹

細胞名稱	人胰腺導(dǎo)管癌細胞； MIA PaCa-2 (STR)
別名	MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2; MiaPaCa
貨號	XF0752
規(guī)格	1×10?cells/T25培養(yǎng)瓶
形態(tài)特征	?上皮細胞樣
生長特性	半貼壁生長
培養(yǎng)基	DMEM+10%FBS+2.5%HS 【MIA PaCa-2培養(yǎng)基有售，請聯(lián)系客服】
凍存條件	無血清細胞凍存液
培養(yǎng)條件	氣相：空氣，95%；CO2,5% 溫度：37℃
傳代方法	1:2傳代；
特征特性	DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS

CRL-1420 MIA PaCa-2 人胰腺癌細胞 的介紹

ATCC® Number: CRL-1420™ Price: $279.00

Designations: MIA PaCa-2

Depositors: A Yunis

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Homo sapiens （human）

Morphology: attached epithelial with floating rounded cells

Source: Organ: pancreas

Disease: carcinoma

Cellular Products: human colony stimulating factor, subclass I （CSF-I）; plasminogen activator

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory per

mits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1975

DNA Profile （STR）: Amelogenin: X

CSF1PO: 10

D13S317: 12,13

D16S539: 10,13

D5S818: 12,13

D7S820: 12,13

THO1: 9,10

TPOX: 9

vWA: 15

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

Isoenzymes: G6PD, B

Age: 65 years

Gender: male

Ethnicity: Caucasian

Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034]

The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034]

Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207

References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line （MIA PaCa-2）. J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455

CRL-1420 MIA PaCa-2 人胰腺癌細胞

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MIA PaCa-2 人胰腺癌細胞 (STR)

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