久久久一区二区四区,女性性高潮国产成人影视,欧美亚洲国产精品久久第一页,亚洲精品aⅴ中文字幕乱码麻豆

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>WISH人羊膜細(xì)胞.

人羊膜細(xì)胞.

簡(jiǎn)要描述:CCL-25 WISH 人羊膜細(xì)胞.,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):WISH
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1926

產(chǎn)品分類(lèi)

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CCL-25 WISH 人羊膜細(xì)胞.

ATCC® Number: CCL-25™ Price: $323.00

Additional information about this cell line

Designations: WISH

Depositors: L Hayflick

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

CCL-25 WISH 人羊膜細(xì)胞.

Source: Organ: HeLa contaminant

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Virus Susceptibility: poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,10

D13S317: 13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Isoenzymes: G6PD, A

HeLa Markers: Y

Comments: This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

The cells are positive for keratin by immunoperoxidase staining.

NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490

26034: . . Am. J. Dis. Child. 103: 460, 1962.




CCL-25 WISH人羊膜細(xì)胞。

ATCC®編號(hào):CCL-25™價(jià)格:323.00美元

有關(guān)此細(xì)胞系的其他信息

名稱(chēng):WISH

存款人:L Hayflick

生物安全級(jí)別:2

運(yùn)送:冷凍

中等和血清:見(jiàn)傳播

生長(zhǎng)特性:貼壁

生物體:智人(人)

形態(tài):上皮

CCL-25 WISH

CCL-25 WISH人羊膜細(xì)胞。

來(lái)源:器官:HeLa污染物

蜂窩產(chǎn)品:角蛋白

許可證/表格:除上述MTA之外,轉(zhuǎn)移此ATCC材料可能需要其他ATCC和/或管理許可證。購(gòu)買(mǎi)ATCC材料的任何人終都需要負(fù)責(zé)獲得許可。請(qǐng)點(diǎn)擊此處了解有關(guān)裝運(yùn)到您所在地的具體要求的信息。

病毒易感性:脊髓灰質(zhì)炎病毒1,2;腺病毒3;水泡性口炎(印第安納州)

反轉(zhuǎn)錄:否定

DNA圖譜(STR):釉原蛋白:X.

CSF1PO:9,10

D13S317:13.3

D16S539:9,10

D5S818:11,12

D7S820:8,12

THO1:7

TPOX:8,12

vWA:16,18

同工酶:G6PD,A

HeLa標(biāo)記:Y

評(píng)論:這條線

zi初被認(rèn)為是源自正常的羊膜,但后來(lái)發(fā)現(xiàn),基于同工酶分析,HeLa標(biāo)記染色體和DNA指紋分析已經(jīng)通過(guò)HeLa細(xì)胞污染建立。

通過(guò)免疫過(guò)氧化物酶染色,細(xì)胞對(duì)角蛋白呈陽(yáng)性。

注意:此行的細(xì)胞含有HeLa標(biāo)記染色體,并且是通過(guò)HeLa污染衍生的

繁殖:ATCC*生長(zhǎng)培養(yǎng)基:Earle's BSS中的低必需培養(yǎng)基(Eagle),含有非必需氨基酸和1mM丙酮酸鈉,90%;胎牛血清,10%

溫度:37.0℃

傳代培養(yǎng):傳代培養(yǎng)比例:建議傳代比例為1:2至1:10

中等更新:每周兩次

取出培養(yǎng)基,加入新鮮的0.25%胰蛋溶液3到5分鐘,去除,讓培養(yǎng)物在室溫下靜置5-10分鐘。加入新鮮培養(yǎng)基,吸入并分裝到新的培養(yǎng)瓶中。

保存:培養(yǎng)基95%; DMSO,5%

相關(guān)產(chǎn)品:推薦培養(yǎng)基(不含ATCC培養(yǎng)基描述的額外補(bǔ)充劑或血清):ATCC 30-2003

推薦血清:ATCC 30-2020

參考文獻(xiàn):22153:Hayflick L.在連續(xù)培養(yǎng)中建立人羊膜細(xì)胞系(WISH)。進(jìn)出口。細(xì)胞研究。 23:14-20,1961。PubMed:13712490

26034:。 。上午。 J. Dis。兒童。 103:460,1962。























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
定边县| 专栏| 永和县| 藁城市| 武安市| 闻喜县| 五莲县| 茌平县| 勃利县| 依兰县| 封开县| 邵武市| 磴口县| 裕民县| 重庆市| 五河县| 丘北县| 唐河县| 房产| 苗栗县| 咸丰县| 都江堰市| 和硕县| 梓潼县| 乐都县| 奎屯市| 梅河口市| 乐昌市| 吉安县| 平罗县| 汾西县| 镇雄县| 年辖:市辖区| 稻城县| 辉县市| 台南县| 明光市| 长寿区| 许昌市| 高邑县| 同德县|